Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by avidin | Rhodococcus rhodochrous | |
additional information | - |
Xanthobacter autotrophicus Py2 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required as stimulatory but not essential cofactor | Xanthobacter autotrophicus Py2 | |
Mg2+ | - |
Rhodobacter capsulatus | |
Mg2+ | required as stimulatory but not essential cofactor | Xanthobacter autotrophicus Py2 | |
Mg2+ | stimulatory effect on enzyme activity | Rhodococcus rhodochrous | |
additional information | - |
Xanthobacter autotrophicus Py2 | |
additional information | dependent on the presence of a divalent metal | Rhodococcus rhodochrous | |
NH4+ | stimulatory effect on enzyme activity | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
1 * 85000, 1 * 74000, 1 * 16000, SDS-PAGE | Rhodococcus rhodochrous |
19600 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
23000 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
68400 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
74000 | - |
1 * 85000, 1 * 74000, 1 * 16000, SDS-PAGE | Rhodococcus rhodochrous |
78300 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
78800 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
85000 | - |
1 * 85000, 1 * 74000, 1 * 16000, SDS-PAGE | Rhodococcus rhodochrous |
85300 | - |
1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Rhodococcus rhodochrous | - |
B276, ATCC 31338, formerly Nocardia corallina | - |
Xanthobacter autotrophicus Py2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose chromatography, but hydrophobic interaction, gel filtration and anion-exchange chromatography by Q-Sepharose resulted in large loss of activity | Rhodococcus rhodochrous |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetone + CO2 + ATP + 2 H2O = acetoacetate + AMP + 2 phosphate | activity is induced in suspensions of acetone- and isopropanol-grown cells | Rhodococcus rhodochrous |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodobacter capsulatus |
additional information | - |
GTP shows best specific activity of tested nucleoside triphosphates: ATP, ITP, XTP, CTP and UTP | Rhodococcus rhodochrous |
0.0015 | 0.003 | cell extracts | Rhodococcus rhodochrous |
0.014 | - |
with ITP as nucleoside triphosphate | Rhodococcus rhodochrous |
0.016 | - |
with GTP as nucleoside triphosphate | Rhodococcus rhodochrous |
0.07 | 0.15 | cell suspension | Rhodococcus rhodochrous |
0.225 | - |
- |
Xanthobacter autotrophicus Py2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetone + CO2 + ATP + H2O | - |
Rhodobacter capsulatus | acetoacetate + AMP + phosphate | - |
? | |
acetone + CO2 + ATP + H2O | - |
Xanthobacter autotrophicus Py2 | acetoacetate + AMP + phosphate | - |
? | |
acetone + CO2 + ATP + H2O | low activity with ATP | Rhodococcus rhodochrous | acetoacetate + AMP + phosphate | - |
? | |
acetone + CO2 + GTP + H2O | better activity than with ATP | Rhodococcus rhodochrous | acetoacetate + GMP + phosphate | - |
? | |
butanone + CO2 + ATP + H2O | - |
Rhodococcus rhodochrous | 3-oxopentanoate + AMP + phosphate | - |
? | |
butanone + CO2 + ATP + H2O | - |
Rhodobacter capsulatus | 3-oxopentanoate + AMP + phosphate | - |
? | |
butanone + CO2 + ATP + H2O | - |
Xanthobacter autotrophicus Py2 | 3-oxopentanoate + AMP + phosphate | - |
? | |
additional information | enzyme expressed by acetone and isopropanol grown cells, not by propylene or glucose grown cells | Rhodococcus rhodochrous | ? | - |
? | |
additional information | enzyme expressed by acetone and isopropanol grown cells, not by propylene or glucose grown cells | Xanthobacter autotrophicus Py2 | ? | - |
? | |
additional information | 2-pentanone, 3-pentanone and 2-hexanone as well as short-chain aliphatic alkanes and alkenes, including propane, are substrates. Several other nucleoside triphosphates are used | Rhodococcus rhodochrous | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 85000, 1 * 74000, 1 * 16000, SDS-PAGE | Rhodococcus rhodochrous |
trimer | 1 * 68400, 1 * 78800, 1 * 23000 by SDS-PAGE and 1 * 78300, 1 * 85300, 1 * 19600 by mass spectrometry. Subunits have an alpha2beta2gamma2 quaternary structure | Xanthobacter autotrophicus Py2 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodococcus rhodochrous |
30 | - |
- |
Xanthobacter autotrophicus Py2 |
30 | - |
assay at | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rhodococcus rhodochrous |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rhodobacter capsulatus | |
ATP | - |
Xanthobacter autotrophicus Py2 | |
ATP | low activity | Rhodococcus rhodochrous | |
GTP | - |
Rhodococcus rhodochrous | |
ITP | - |
Rhodococcus rhodochrous | |
additional information | nucleoside triphosphate required, ATP does not support acetone carboxylation | Rhodococcus rhodochrous |